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EFHA2 Rabbit pAb
EFHA2 Rabbit pAb
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Application 
| IHC-P, IHC-F, IF, ICC |
|---|---|
| Primary Accession | Q86XE3 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 61 KDa |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human EFHA2 |
| Epitope Specificity | 451-530/530 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Mitochondrion (By similarity). Membrane; Single-pass membrane protein (Potential). |
| SIMILARITY | Contains 2 EF-hand domains. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Gene ID | 286097 |
|---|---|
| Other Names | Calcium uptake protein 3, mitochondrial, hMICU3, EF-hand domain-containing family member A2, MICU3 {ECO:0000303|PubMed:30699349, ECO:0000312|HGNC:HGNC:27820} |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500 |
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃. |
| Name | MICU3 {ECO:0000303|PubMed:30699349, ECO:0000312|HGNC:HGNC:27820} |
|---|---|
| Function | Tissue-specific calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which specifically regulates MCU channel activity in the central nervous system and skeletal muscle (PubMed:29725115). Senses calcium level via its EF-hand domains: compared to MICU1 and MICU2, MICU3 has a higher affinity for calcium (PubMed:29725115). MICU1 and MICU3 form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium (PubMed:29725115). At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake (PubMed:29725115). At higher calcium levels, calcium- binding to MICU1 and MICU3 induces a conformational change that weakens MCU-MICU1 interactions and moves the MICU1-MICU3 heterodimer away from the pore, allowing calcium permeation through the MCU channel (PubMed:29725115). The high calcium affinity of MICU3 lowers the calcium threshold necessary for calcium permeation through the MCU channel (PubMed:29725115). The MICU1-MICU3 heterodimer promotes flexibility of neurotransmission in neuronal cells by enhancing mitochondrial calcium uptake in presynapses (PubMed:29725115). It is also required to increase mitochondrial calcium uptake in skeletal muscle cells, thereby increasing ATP production (By similarity). |
| Cellular Location | Mitochondrion intermembrane space {ECO:0000250|UniProtKB:Q8IYU8}. Mitochondrion inner membrane {ECO:0000250|UniProtKB:Q8IYU8}. Note=Recruited to the mitochondrial inner membrane via its association with the uniplex complex {ECO:0000250|UniProtKB:Q8IYU8} |
| Tissue Location | Specifically expressed in the central nervous system and skeletal muscle. |
Research Areas
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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Cat# AP94841
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